Sure, it may sit in the depths of HR until the end of time, but you can be sure of two things: One, your former boss (and future reference) will read it and be impressed. And two, if you ever decide to come back to your company (hey, it happens), it's a very good thing that last thing on file is a great, professionally written resignation letter.
The Thank You. Next, it's always a good idea to thank your employer for the opportunity, describing some of the key things you've enjoyed and learned on the job. And yes, this is true even if you're thrilled to be leaving. Remember__you may need these people for a reference down the line, and leaving things on a good note will leave a lasting (positive) impression.